Capillary Electrophoresis methods
There are several different forms of capillary electrophoresis, each of which has its own particular advantage. Several of these methods are briefly described.
- Capillary Zone Electrophoresis
- Capillary gel electrophoresis
- Capillary Electrochromatography
Capillary Zone electrophoresis
Capillary Zone electrophoresis also known as free solution capillary electrophoresis.
A mixture in a solution can be separated into its individual components quickly and easily. The separation is based on the differences in electrophoretic mobility which is directly proportional to the charge on molecule and inversely proportional to the viscosity of the solvent and radius of the atom. The velocity at which the ion is directly proportional to the electrophoretic mobility and the magnitude of the electric field.
The fused silica capillaries have silanol groups that become ionized in the buffer. The negatively charged SiO- ions attract positively charged cations which forms two layers. A fixed layer and a diffused cation layer.
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